6ROH

Cryo-EM structure of the autoinhibited Drs2p-Cdc50p

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable phospholipid-transporting ATPase DRS2	polymer	1460	163730.8	1	UniProt (P39524) Pfam (PF16209) Pfam (PF13246) Pfam (PF16212)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (C)	Cell division control protein 50	polymer	413	47371.8	1	UniProt (P25656) Pfam (PF03381)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	C (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	D (D)	beta-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G31886NL)
5	E (A)	1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE	non-polymer		455.4	1	Chemie (PSF)
6	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	G (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	H (A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A: 1 - 1355 (UniProt: P39524)

PDB	External Database	Details
Gly 1356	-	expression tag
Gly 1357	-	expression tag
Gly 1358	-	expression tag
Gly 1359	-	expression tag
Leu 1360	-	expression tag
Val 1361	-	expression tag
Pro 1362	-	expression tag
Arg 1363	-	expression tag
Gly 1364	-	expression tag
Ser 1365	-	expression tag
Gly 1366	-	expression tag
Gly 1367	-	expression tag
Thr 1368	-	expression tag
Ala 1369	-	expression tag
Ala 1370	-	expression tag
Ala 1371	-	expression tag
Pro 1372	-	expression tag
Gly 1373	-	expression tag
Pro 1374	-	expression tag
Ala 1375	-	expression tag
Pro 1376	-	expression tag
Ala 1377	-	expression tag
Pro 1378	-	expression tag
Ala 1379	-	expression tag
Pro 1380	-	expression tag
Ala 1381	-	expression tag
Ser 1382	-	expression tag
Ala 1383	-	expression tag
Pro 1384	-	expression tag
Ala 1385	-	expression tag
Ala 1386	-	expression tag
Ala 1387	-	expression tag
Ala 1388	-	expression tag
Pro 1389	-	expression tag
Ala 1390	-	expression tag
Gly 1391	-	expression tag
Ala 1392	-	expression tag
Gly 1393	-	expression tag
Thr 1394	-	expression tag
Pro 1395	-	expression tag
Val 1396	-	expression tag
Thr 1397	-	expression tag
Ala 1398	-	expression tag
Pro 1399	-	expression tag
Leu 1400	-	expression tag
Ala 1401	-	expression tag
Gly 1402	-	expression tag
Thr 1403	-	expression tag
Ile 1404	-	expression tag
Trp 1405	-	expression tag
Lys 1406	-	expression tag
Val 1407	-	expression tag
Leu 1408	-	expression tag
Ala 1409	-	expression tag
Ser 1410	-	expression tag
Glu 1411	-	expression tag
Gly 1412	-	expression tag
Gln 1413	-	expression tag
Thr 1414	-	expression tag
Val 1415	-	expression tag
Ala 1416	-	expression tag
Ala 1417	-	expression tag
Gly 1418	-	expression tag
Glu 1419	-	expression tag
Val 1420	-	expression tag
Leu 1421	-	expression tag
Leu 1422	-	expression tag
Ile 1423	-	expression tag
Leu 1424	-	expression tag
Glu 1425	-	expression tag
Ala 1426	-	expression tag
Met 1427	-	expression tag
Lys 1428	-	expression tag
Met 1429	-	expression tag
Glu 1430	-	expression tag
Thr 1431	-	expression tag
Glu 1432	-	expression tag
Ile 1433	-	expression tag
Arg 1434	-	expression tag
Ala 1435	-	expression tag
Ala 1436	-	expression tag
Gln 1437	-	expression tag
Ala 1438	-	expression tag
Gly 1439	-	expression tag
Thr 1440	-	expression tag
Val 1441	-	expression tag
Arg 1442	-	expression tag
Gly 1443	-	expression tag
Ile 1444	-	expression tag
Ala 1445	-	expression tag
Val 1446	-	expression tag
Lys 1447	-	expression tag
Ala 1448	-	expression tag
Gly 1449	-	expression tag
Asp 1450	-	expression tag
Ala 1451	-	expression tag
Val 1452	-	expression tag
Ala 1453	-	expression tag
Val 1454	-	expression tag
Gly 1455	-	expression tag
Asp 1456	-	expression tag
Thr 1457	-	expression tag
Leu 1458	-	expression tag
Met 1459	-	expression tag
Thr 1460	-	expression tag

C: 1 - 391 (UniProt: P25656)

PDB	External Database	Details
Gly 392	-	expression tag
Gly 393	-	expression tag
Gly 394	-	expression tag
Gly 395	-	expression tag
Leu 396	-	expression tag
Val 397	-	expression tag
Pro 398	-	expression tag
Arg 399	-	expression tag
Gly 400	-	expression tag
Ser 401	-	expression tag
Gly 402	-	expression tag
Gly 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	211102.6
Branched	Number of molecules	2
	Total formula weight	1173.1
Non-Polymers*	Number of molecules	3
	Total formula weight	701.0
All*	Total formula weight	212976.6

*Water molecules are not included.