6RME

Structure of IMP bound Plasmodium falciparum IMP-nucleotidase mutant D172N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G (A, G)	IMP-specific 5'-nucleotidase, putative	polymer	384	44955.8	2	UniProt (A0A144A134) Pfam (PF06437)	Plasmodium falciparum (isolate 3D7)
2	B, D (B, D)	IMP-specific 5'-nucleotidase, putative	polymer	385	45084.0	2	UniProt (A0A144A134) Pfam (PF06437)	Plasmodium falciparum (isolate 3D7)
3	C (C)	IMP-specific 5'-nucleotidase, putative	polymer	394	46116.2	1	UniProt (A0A144A134) Pfam (PF06437)	Plasmodium falciparum (isolate 3D7)
4	E, H (E, H)	IMP-specific 5'-nucleotidase, putative	polymer	386	45198.1	2	UniProt (A0A144A134) Pfam (PF06437)	Plasmodium falciparum (isolate 3D7)
5	F (F)	IMP-specific 5'-nucleotidase, putative	polymer	388	45444.4	1	UniProt (A0A144A134) Pfam (PF06437)	Plasmodium falciparum (isolate 3D7)
6	I, K, M, O, R... (A, B, C, D, E...)	INOSINIC ACID	non-polymer		348.2	8	Chemie (IMP)
7	J, L, N, P, S... (A, B, C, D, E...)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
8	Q, T (D, E)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
9	AA, BA, CA (B, C, F)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

A, G: 47 - 430 (UniProt: A0A144A134)

PDB	External Database	Details
Asn 172	Asp 172	engineered mutation

B, D: 46 - 430 (UniProt: A0A144A134)

PDB	External Database	Details
Asn 172	Asp 172	engineered mutation

C: 38 - 431 (UniProt: A0A144A134)

PDB	External Database	Details
Asn 172	Asp 172	engineered mutation

E, H: 46 - 431 (UniProt: A0A144A134)

PDB	External Database	Details
Asn 172	Asp 172	engineered mutation

F: 45 - 431 (UniProt: A0A144A134)

PDB	External Database	Details
Phe 44	-	expression tag
Asn 172	Asp 172	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	362036.3
Non-Polymers*	Number of molecules	18
	Total formula weight	3164.3
All*	Total formula weight	365200.5

*Water molecules are not included.