6RJA
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | AcrIIA6 | polymer | 183 | 21464.4 | 2 | UniProt (A0A2U7VKE8) | Streptococcus phage D1811 | |
| 2 | C, F (C, F) | CRISPR-associated endonuclease Cas9 1 | polymer | 1121 | 129701.0 | 2 | UniProt (Q03LF7) Pfam (PF18470) Pfam (PF13395) Pfam (PF18541) Pfam (PF18061) Pfam (PF18070) | Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) | CRISPR-associated endonuclease Cas9 |
| 3 | D, G (D, G) | RNA (78-MER) | polymer | 117 | 37438.0 | 2 | Streptococcus thermophilus | ||
| 4 | E, H (E, H) | tDNA20 | polymer | 20 | 6101.0 | 2 | Streptococcus virus 2972 |
Sequence modifications
C, F: 1 - 1121 (UniProt: Q03LF7)
| PDB | External Database | Details |
|---|---|---|
| Thr 56 | Ala 56 | conflict |
| Ile 132 | Val 132 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 389408.8 | |
| All* | Total formula weight | 389408.8 |
