6RJ9
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | AcrIIA6 | polymer | 183 | 21464.4 | 2 | UniProt (A0A2U7VKE8) In PDB | Streptococcus phage D1811 | |
2 | C | CRISPR-associated endonuclease Cas9 1 | polymer | 1121 | 129701.0 | 1 | UniProt (Q03LF7) Pfam (PF18470) Pfam (PF13395) Pfam (PF18541) Pfam (PF18061) Pfam (PF18070) In PDB | Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) | CRISPR-associated endonuclease Cas9 |
3 | D | sgRNA | polymer | 117 | 37438.0 | 1 | Streptococcus thermophilus | ||
4 | E | tDNA20 | polymer | 20 | 6101.0 | 1 | Streptococcus virus 2972 |
Sequence modifications
C: 1 - 1121 (UniProt: Q03LF7)
PDB | External Database | Details |
---|---|---|
Thr 56 | Ala 56 | conflict |
Ile 132 | Val 132 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 216168.8 | |
All* | Total formula weight | 216168.8 |