6RH3

Cryo-EM structure of E. coli RNA polymerase elongation complex bound to CTP substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	N	Non-template DNA	polymer	39	12064.7	1		Escherichia coli K-12
2	T	Template DNA	polymer	39	11864.6	1		Escherichia coli K-12
3	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	D	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	E	DNA-directed RNA polymerase subunit omega	polymer	73	8321.4	1	UniProt (L4IY67) In PDB	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
7	R	RNA	polymer	14	4503.7	1		Escherichia coli K-12
8	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
9	D	CYTIDINE-5'-TRIPHOSPHATE	non-polymer		483.2	1	Chemie (CTP)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	416059.5
Non-Polymers*	Number of molecules	4
	Total formula weight	638.3
All*	Total formula weight	416697.8

*Water molecules are not included.