6RF4
Crystal structure of the potassium-pumping S254A mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Sodium pumping rhodopsin | polymer | 288 | 32595.6 | 5 | UniProt (N0DKS8) Pfam (PF01036) | Dokdonia eikasta | |
| 2 | AB, BA, CA, EA, F... (D, C, A, E, B) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 25 | Chemie (OLC) | |||
| 3 | BB, CB, DA, DB, EB... (D, C, A, E, B) | EICOSANE | non-polymer | 282.5 | 29 | Chemie (LFA) | |||
| 4 | HB, N, SB, UA, Y (D, A, E, C, B) | SODIUM ION | non-polymer | 23.0 | 5 | Chemie (NA) | |||
| 5 | IB, O, TB, VA, Z (D, A, E, C, B) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 5 | Chemie (BOG) | |||
| 6 | AA, JB, R, VB, XA (B, D, A, E, C) | RETINAL | non-polymer | 284.4 | 5 | Chemie (RET) | |||
| 7 | AC, WB, XB, YB, ZB (E, A, B, C, D) | water | water | 18.0 | 328 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 1 - 280 (UniProt: N0DKS8)
| PDB | External Database | Details |
|---|---|---|
| Ala 254 | Ser 254 | engineered mutation |
| Leu 281 | - | expression tag |
| Glu 282 | - | expression tag |
| His 283 | - | expression tag |
| His 284 | - | expression tag |
| His 285 | - | expression tag |
| His 286 | - | expression tag |
| His 287 | - | expression tag |
| His 288 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 162977.9 | |
| Non-Polymers* | Number of molecules | 69 |
| Total formula weight | 20106.3 | |
| All* | Total formula weight | 183084.2 |
