6RF4

Crystal structure of the potassium-pumping S254A mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Sodium pumping rhodopsin	polymer	288	32595.6	5	UniProt (N0DKS8) Pfam (PF01036) In PDB	Dokdonia eikasta
2	D, C, A, E, B	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	25	Chemie (OLC)
3	D, C, A, E, B	EICOSANE	non-polymer		282.5	29	Chemie (LFA)
4	D, A, E, C, B	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
5	D, A, E, C, B	octyl beta-D-glucopyranoside	non-polymer		292.4	5	Chemie (BOG)
6	B, D, A, E, C	RETINAL	non-polymer		284.4	5	Chemie (RET)
7		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 280 (UniProt: N0DKS8)

PDB	External Database	Details
Ala 254	Ser 254	engineered mutation
Leu 281	-	expression tag
Glu 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	162977.9
Non-Polymers*	Number of molecules	69
	Total formula weight	20106.3
All*	Total formula weight	183084.2

*Water molecules are not included.