6RF3

Crystal structure of the potassium-pumping G263F mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Sodium pumping rhodopsin	polymer	288	32969.1	5	UniProt (N0DKS8) Pfam (PF01036) In PDB	Dokdonia eikasta
2	B, C, A, E, D	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	60	Chemie (OLC)
3	D, B, E, C, A	EICOSANE	non-polymer		282.5	33	Chemie (LFA)
4	E, D, C, B, A	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
5	E, D, C, B, A	octyl beta-D-glucopyranoside	non-polymer		292.4	5	Chemie (BOG)
6		water	water		18.0	462	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 280 (UniProt: N0DKS8)

PDB	External Database	Details
Phe 263	Gly 263	engineered mutation
Leu 281	-	expression tag
Glu 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag
His 287	-	expression tag
His 288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164845.6
Non-Polymers*	Number of molecules	103
	Total formula weight	32293.2
All*	Total formula weight	197138.9

*Water molecules are not included.