6RDT

Cryo-EM structure of Polytomella F-ATP synthase, Rotary substate 1E, composite map

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	0	ASA-10: Polytomella F-ATP synthase associated subunit 10	polymer	82	8731.9	1	UniProt (by SIFTS) (A0A5H1ZR95) In PDB	Polytomella sp. Pringsheim 198.80
2	1	ATP synthase associated protein ASA1	polymer	618	68679.9	1	UniProt (Q85JD5) In PDB	Polytomella sp. Pringsheim 198.80
3	2	ASA-2: Polytomella F-ATP synthase associated subunit 2	polymer	441	44842.1	1	UniProt (by SIFTS) (D7P6B9) In PDB	Polytomella sp. Pringsheim 198.80
4	3	Mitochondrial F1F0 ATP synthase associated 32 kDa protein	polymer	325	34850.4	1	UniProt (K0J903) In PDB	Polytomella sp. Pringsheim 198.80
5	4	Mitochondrial ATP synthase associated protein ASA4	polymer	294	31275.1	1	UniProt (D7NIZ2) In PDB	Polytomella sp. Pringsheim 198.80
6	5	Mitochondrial F1F0 ATP synthase associated 14 kDa protein	polymer	123	14004.4	1	UniProt (A0A024FSR7) In PDB	Polytomella sp. Pringsheim 198.80
7	6	Mitochondrial ATP synthase subunit ASA6	polymer	151	15904.3	1	UniProt (D7P897) In PDB	Polytomella sp. Pringsheim 198.80
8	7	Mitochondrial ATP synthase associated protein ASA7	polymer	190	20553.2	1	UniProt (D8V7I2) In PDB	Polytomella sp. Pringsheim 198.80
9	8	Mitochondrial ATP synthase subunit ASA8	polymer	89	9883.4	1	UniProt (D8V7I7) In PDB	Polytomella sp. Pringsheim 198.80
10	9	ASA-9: Polytomella F-ATP synthase associated subunit 9	polymer	97	11001.7	1	UniProt (by SIFTS) (A0A5H1ZR73) In PDB	Polytomella sp. Pringsheim 198.80
11	A, B, C, D, E...	Mitochondrial ATP synthase subunit c	polymer	127	12664.0	10	UniProt (D7P7X5) Pfam (PF00137) In PDB	Polytomella sp. Pringsheim 198.80
12	M	Mitochondrial ATP synthase subunit 6	polymer	327	34802.3	1	UniProt (H8PGG3) Pfam (PF00119) In PDB	Polytomella sp. Pringsheim 198.80
13	P	Mitochondrial ATP synthase subunit OSCP	polymer	229	25530.8	1	UniProt (D8V7I1) Pfam (PF00213) In PDB	Polytomella sp. Pringsheim 198.80
14	Q	epsilon: Polytomella F-ATP synthase epsilon subunit	polymer	74	8205.5	1	Pfam (PF04627) UniProt (by SIFTS) (A0A5H1ZR74) In PDB	Polytomella sp. Pringsheim 198.80
15	R	Mitochondrial ATP synthase subunit delta	polymer	199	20880.5	1	UniProt (D7P7X6) Pfam (PF02823) In PDB	Polytomella sp. Pringsheim 198.80
16	S	ATP synthase gamma chain, mitochondrial	polymer	317	34639.6	1	UniProt (Q4LDE7) Pfam (PF00231) In PDB	Polytomella sp. Pringsheim 198.80
17	T, U, V	ATP synthase subunit alpha	polymer	562	60766.2	3	UniProt (A0ZW40) Pfam (PF02874) Pfam (PF00006) Pfam (PF00306) In PDB	Polytomella sp. Pringsheim 198.80
18	X, Y, Z	ATP synthase subunit beta	polymer	574	61939.1	3	UniProt (A0ZW41) Pfam (PF02874) Pfam (PF00006) In PDB	Polytomella sp. Pringsheim 198.80
19	M	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
20	T, U, V	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
21	T, U, V, X, Y	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
22	X, Y	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

T, U, V: 1 - 562 (UniProt: A0ZW40)

PDB	External Database	Details
Arg 266	Lys 266	conflict

X, Y, Z: 1 - 574 (UniProt: A0ZW41)

PDB	External Database	Details
Ala 350	Gly 350	conflict
Leu 387	Arg 387	conflict

Contents of the asymmetric unit

Polymers	Number of chains	31
	Total formula weight	878540.8
Non-Polymers*	Number of molecules	11
	Total formula weight	2562.9
All*	Total formula weight	881103.7

*Water molecules are not included.