6R7I
Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | COP9 signalosome complex subunit 1 | polymer | 462 | 52494.1 | 1 | UniProt (Q13098) Pfam (PF10602) Pfam (PF01399) Pfam (PF21151) | Homo sapiens (Human) | Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH |
| 2 | B (B) | COP9 signalosome complex subunit 2 | polymer | 443 | 51664.6 | 1 | UniProt (P61201) Pfam (PF01399) | Homo sapiens (Human) | Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TRIP-15 |
| 3 | C (C) | COP9 signalosome complex subunit 3 | polymer | 401 | 45606.5 | 1 | UniProt (Q9UNS2) Pfam (PF22788) Pfam (PF01399) Pfam (PF21215) | Homo sapiens (Human) | Signalosome subunit 3,JAB1-containing signalosome subunit 3 |
| 4 | D (D) | COP9 signalosome complex subunit 4 | polymer | 407 | 46479.9 | 1 | UniProt (Q9BT78) Pfam (PF18420) Pfam (PF22241) Pfam (PF01399) | Homo sapiens (Human) | Signalosome subunit 4,JAB1-containing signalosome subunit 4 |
| 5 | E (E) | COP9 signalosome complex subunit 5 | polymer | 334 | 37621.7 | 1 | UniProt (Q92905) Pfam (PF01398) Pfam (PF18323) | Homo sapiens (Human) | Signalosome subunit 5,Jun activation domain-binding protein 1 |
| 6 | F (F) | COP9 signalosome complex subunit 6 | polymer | 327 | 36203.4 | 1 | UniProt (Q7L5N1) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (Human) | Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP |
| 7 | G (G) | COP9 signalosome complex subunit 7b | polymer | 215 | 23944.4 | 1 | UniProt (Q9H9Q2) Pfam (PF22061) Pfam (PF01399) Pfam (PF18392) | Homo sapiens (Human) | Signalosome subunit 7b,JAB1-containing signalosome subunit 7b |
| 8 | H (H) | COP9 signalosome complex subunit 8 | polymer | 209 | 23245.5 | 1 | UniProt (Q99627) Pfam (PF10075) | Homo sapiens (Human) | Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8 |
| 9 | I (N) | NEDD8 | polymer | 78 | 8876.8 | 1 | Pfam (PF00240) UniProt (by SIFTS) (Q15843) | Homo sapiens (Human) | |
| 10 | J (O) | Cullin-2 | polymer | 745 | 87098.9 | 1 | UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) | Homo sapiens (Human) | CUL-2 |
| 11 | K (P) | Elongin-B | polymer | 106 | 11973.6 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (Human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 12 | L (Q) | Elongin-C | polymer | 99 | 11338.8 | 1 | Pfam (PF03931) UniProt (by SIFTS) (Q03071) | Homo sapiens | |
| 13 | M (R) | E3 ubiquitin-protein ligase RBX1 | polymer | 85 | 10021.6 | 1 | Pfam (PF12678) UniProt (by SIFTS) (P62877) | Homo sapiens | |
| 14 | N, O, P, Q (E, R) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 15 | R, S, T (A, P, Q) | water | water | 18.0 | 169 | Chemie (HOH) |
Sequence modifications
A: 77 - 527 (UniProt: Q13098)
D: 1 - 406 (UniProt: Q9BT78)
G: 1 - 215 (UniProt: Q9H9Q2)
| PDB | External Database | Details |
|---|---|---|
| Lys 70 | - | expression tag |
| Val 71 | - | expression tag |
| Tyr 72 | - | expression tag |
| Ile 73 | - | expression tag |
| Glu 74 | - | expression tag |
| Lys 75 | - | expression tag |
| Asp 76 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 167 | Ile 167 | conflict |
| Ser 168 | Asn 168 | conflict |
| Ala 169 | Asn 169 | conflict |
| Ala 171 | Val 171 | conflict |
| Arg 172 | Lys 172 | conflict |
| Gln 175 | His 175 | conflict |
| Val 179 | Asp 179 | conflict |
| Val 183 | Ala 183 | conflict |
| Ser 186 | Leu 186 | conflict |
| Glu 190 | Gln 190 | conflict |
| Ser 193 | Leu 193 | conflict |
| His 198 | Tyr 198 | conflict |
| Gln 201 | Asn 201 | conflict |
| Gln 202 | His 202 | conflict |
| Leu 203 | Asn 203 | conflict |
| Gly 204 | Arg 204 | conflict |
| Leu 205 | Thr 205 | conflict |
| Lys 206 | Gln 206 | conflict |
| Ile 209 | Val 209 | conflict |
| Ser 211 | Ala 211 | conflict |
| Ala 214 | Thr 214 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 446569.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 446831.5 |
