6R6H

Structural basis of Cullin-2 RING E3 ligase regulation by the COP9 signalosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	COP9 signalosome complex subunit 1	polymer	491	55606.5	1	UniProt (Q13098) Pfam (PF10602) Pfam (PF01399) Pfam (PF21151) In PDB	Homo sapiens (Human)	Signalosome subunit 1,G protein pathway suppressor 1,GPS-1,JAB1-containing signalosome subunit 1,Protein MFH
2	B	COP9 signalosome complex subunit 2	polymer	443	51664.6	1	UniProt (P61201) Pfam (PF01399) In PDB	Homo sapiens (Human)	Signalosome subunit 2,Alien homolog,JAB1-containing signalosome subunit 2,Thyroid receptor-interacting protein 15,TRIP-15
3	C	COP9 signalosome complex subunit 3	polymer	403	45808.8	1	UniProt (Q9UNS2) Pfam (PF22788) Pfam (PF01399) Pfam (PF21215) In PDB	Homo sapiens (Human)	Signalosome subunit 3,JAB1-containing signalosome subunit 3
4	D	COP9 signalosome complex subunit 4	polymer	406	46322.7	1	UniProt (Q9BT78) Pfam (PF18420) Pfam (PF22241) Pfam (PF01399) In PDB	Homo sapiens (Human)	Signalosome subunit 4,JAB1-containing signalosome subunit 4
5	E	COP9 signalosome complex subunit 5	polymer	334	37621.7	1	UniProt (Q92905) Pfam (PF01398) Pfam (PF18323) In PDB	Homo sapiens (Human)	Signalosome subunit 5,Jun activation domain-binding protein 1
6	F	COP9 signalosome complex subunit 6	polymer	308	34713.8	1	UniProt (Q7L5N1) Pfam (PF01398) Pfam (PF13012) In PDB	Homo sapiens (Human)	Signalosome subunit 6,JAB1-containing signalosome subunit 6,MOV34 homolog,Vpr-interacting protein,hVIP
7	H	COP9 signalosome complex subunit 8	polymer	209	23245.5	1	UniProt (Q99627) Pfam (PF10075) In PDB	Homo sapiens (Human)	Signalosome subunit 8,COP9 homolog,hCOP9,JAB1-containing signalosome subunit 8
8	O	Cullin-2	polymer	745	87098.9	1	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (Human)	CUL-2
9	P	Elongin-B	polymer	105	11819.5	1	UniProt (Q15370) Pfam (PF00240) In PDB	Homo sapiens (Human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
10	Q	ELOC_HUMAN	polymer	99	11338.8	1	Pfam (PF03931) UniProt (by SIFTS) (Q03071) In PDB	Homo sapiens (Human)
11	R	RBX1_HUMAN	polymer	86	10092.6	1	Pfam (PF12678) UniProt (by SIFTS) (P62877) In PDB	Homo sapiens (Human)
12	V	von Hippel-Lindau disease tumor suppressor	polymer	150	17330.8	1	UniProt (P40337) Pfam (PF01847) Pfam (PF17211) In PDB	Homo sapiens (Human)	Protein G7,pVHL
13	G	COP9 signalosome complex subunit 7b	polymer	206	23228.5	1	UniProt (Q9H9Q2) Pfam (PF22061) Pfam (PF01399) Pfam (PF18392) In PDB	Homo sapiens (Human)	Signalosome subunit 7b,JAB1-containing signalosome subunit 7b
14	E	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

C: 1 - 383 (UniProt: Q9UNS2)

PDB	External Database	Details
Ile 384	-	expression tag
Glu 385	-	expression tag
Leu 386	-	expression tag
Asp 387	-	expression tag
Glu 388	-	expression tag
Arg 389	-	expression tag
Leu 390	-	expression tag
Lys 391	-	expression tag
Ala 392	-	expression tag
Met 393	-	expression tag
Asp 394	-	expression tag
Gln 395	-	expression tag
Glu 396	-	expression tag
Ile 397	-	expression tag
Thr 398	-	expression tag
Val 399	-	expression tag
Asn 400	-	expression tag
Pro 401	-	expression tag
Gln 402	-	expression tag
Phe 403	-	expression tag

P: 1 - 104 (UniProt: Q15370)

PDB	External Database	Details
Ala 105	-	expression tag

V: 60 - 207 (UniProt: P40337)

PDB	External Database	Details
Ala 208	-	expression tag
Ala 209	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	455892.8
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	455958.2

*Water molecules are not included.