6R64

N-terminal domain of modification dependent EcoKMcrA restriction endonuclease (NEco) in complex with C5mCGG target sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	5-methylcytosine-specific restriction enzyme A	polymer	152	17368.6	2	UniProt (P24200)	Escherichia coli K12	EcoKMcrA
2	C, E (C, E)	DNA (5'-D(*TP*CP*AP*CP*(5CM)P*GP*GP*TP*TP*C)-3')	polymer	10	3010.0	2		synthetic construct
3	D, F (D, F)	DNA (5'-D(*GP*AP*AP*CP*(5CM)P*GP*GP*TP*GP*A)-3')	polymer	10	3108.1	2		synthetic construct
4	G, H, I, J, K... (A, B, C, D, E...)	water	water		18.0	146	Chemie (HOH)

Sequence modifications

A, B: 1 - 143 (UniProt: P24200)

PDB	External Database	Details
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	46973.3
All*	Total formula weight	46973.3

*Water molecules are not included.