6R5R
The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with cellobiose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Periplasmic beta-glucosidase | polymer | 733 | 80154.9 | 1 | UniProt (Q9I311) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | |
| 2 | B (A) | Periplasmic beta-glucosidase | polymer | 734 | 80226.0 | 1 | UniProt (Q9I311) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | |
| 3 | C, D (C, D) | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
| 4 | E, F, G, H, L... (B, A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 7 | Chemie (PEG) | |||
| 5 | I, J (B) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
| 6 | K, O (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | P, Q (B, A) | water | water | 18.0 | 1409 | Chemie (HOH) |
Sequence modifications
B: 32 - 764 (UniProt: Q9I311)
A: 31 - 764 (UniProt: Q9I311)
| PDB | External Database | Details |
|---|---|---|
| Asn 286 | Asp 286 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 286 | Asp 286 | engineered mutation |
| - | Asp 621 | deletion |
| Asp 621 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 160380.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1091.8 | |
| All* | Total formula weight | 162157.3 |
