6R5R

The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with cellobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Periplasmic beta-glucosidase	polymer	733	80154.9	1	UniProt (Q9I311) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
2	A	Periplasmic beta-glucosidase	polymer	734	80226.0	1	UniProt (Q9I311) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
3	C, D	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO)		beta-cellobiose
4	B, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	7	Chemie (PEG)
5	B	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
6	B, A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7		water	water		18.0	1409	Chemie (HOH)

Sequence modifications

B: 32 - 764 (UniProt: Q9I311)

PDB	External Database	Details
Asn 286	Asp 286	engineered mutation

A: 31 - 764 (UniProt: Q9I311)

PDB	External Database	Details
Asn 286	Asp 286	engineered mutation
-	Asp 621	deletion
Asp 621	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	160380.9
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	11
	Total formula weight	1091.8
All*	Total formula weight	162157.3

*Water molecules are not included.