6R5C

Crystal structure of PPEP-1(W103F/E143A/Y178F) in complex with substrate peptide Ac-EVNPPVP-CONH2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Pro-Pro endopeptidase	polymer	198	21870.6	2	UniProt (Q183R7) Pfam (PF07737) In PDB	Peptoclostridium difficile	PPEP-1,Zinc metalloprotease Zmp1
2	C, D	ACE-GLU-VAL-ASN-PRO-PRO-VAL-LPD	polymer	8	775.9	2		Clostridioides difficile
3	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4		water	water		18.0	322	Chemie (HOH)

Sequence modifications

A, B: 27 - 220 (UniProt: Q183R7)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Phe 103	Trp 103	engineered mutation
Ala 143	Glu 143	engineered mutation
Phe 178	Tyr 178	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45293.0
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	45423.8

*Water molecules are not included.