6R3U

Endo-levanase BT1760 mutant E221A from Bacteroides thetaiotaomicron complexed with levantetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycoside hydrolase family 32	polymer	508	57860.0	1	UniProt (Q8A6W6) Pfam (PF00251) Pfam (PF16346) In PDB	Bacteroides thetaiotaomicron
2	B	beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose-(2-6)-beta-D-fructofuranose	branched		666.6	1	In PDB GlyTouCan (G94951WG)
3	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5		water	water		18.0	326	Chemie (HOH)

Sequence modifications

A: 2 - 502 (UniProt: Q8A6W6)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 221	Glu 242	engineered mutation
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag
His 508	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	57860.0
Branched	Number of molecules	1
	Total formula weight	666.6
Non-Polymers*	Number of molecules	5
	Total formula weight	407.1
All*	Total formula weight	58933.7

*Water molecules are not included.