6QTA

Crystal structure of Rea1-MIDAS/Rsa4-UBL complex from Chaetomium thermophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Midasin,Midasin	polymer	280	31464.8	1	UniProt (G0SHE6) In PDB	Chaetomium thermophilum var. thermophilum DSM 1495
2	B	Ribosome assembly protein 4	polymer	108	12323.8	1	UniProt (G0SC29) In PDB	Chaetomium thermophilum var. thermophilum DSM 1495	Notchless protein homolog 1,Ribosome biogenesis factor RSA4
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6		water	water		18.0	219	Chemie (HOH)

Sequence modifications

A: 4690 - 4733 (UniProt: G0SHE6)

PDB	External Database	Details
Met 4689	-	initiating methionine

A: 4737 - 4997 (UniProt: G0SHE6)

PDB	External Database	Details
Gly 4734	-	linker
Ser 4735	-	linker
Gly 4736	-	linker
Gly 4998	-	expression tag
Ser 4999	-	expression tag
His 5000	-	expression tag
His 5001	-	expression tag
His 5002	-	expression tag
His 5003	-	expression tag
His 5004	-	expression tag
His 5005	-	expression tag

B: 30 - 128 (UniProt: G0SC29)

PDB	External Database	Details
Met 21	-	initiating methionine
Lys 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
Pro 29	-	expression tag
Met 30	Val 30	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43788.6
Non-Polymers*	Number of molecules	11
	Total formula weight	957.2
All*	Total formula weight	44745.8

*Water molecules are not included.