6QSY
Engineered streptavidin variant (H--WY) in complex with the Strep-tag II peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Streptavidin | polymer | 125 | 13299.4 | 1 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | B (P) | Strep-tag II peptide | polymer | 12 | 1335.4 | 1 | synthetic construct | ||
| 3 | C, E, F (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
| 4 | D (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 5 | G, H (A, P) | water | water | 18.0 | 70 | Chemie (HOH) |
Sequence modifications
A: 14 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Met 13 | - | initiating methionine |
| Val 44 | Glu 68 | conflict |
| Thr 45 | Ser 69 | conflict |
| Arg 47 | Val 71 | conflict |
| His 117 | Ala 141 | engineered mutation |
| - | Asn 142 | deletion |
| - | Ala 143 | deletion |
| Tyr 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 14634.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 572.7 | |
| All* | Total formula weight | 15207.5 |
