6QSY
Engineered streptavidin variant (H--WY) in complex with the Strep-tag II peptide
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Streptavidin | polymer | 125 | 13299.4 | 1 | UniProt (P22629) Pfam (PF01382) In PDB | Streptomyces avidinii | |
2 | P | Strep-tag II peptide | polymer | 12 | 1335.4 | 1 | synthetic construct | ||
3 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 3 | Chemie (PGE) | |||
4 | A | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
5 | water | water | 18.0 | 70 | Chemie (HOH) |
Sequence modifications
A: 14 - 139 (UniProt: P22629)
PDB | External Database | Details |
---|---|---|
Met 13 | - | initiating methionine |
Val 44 | Glu 68 | conflict |
Thr 45 | Ser 69 | conflict |
Arg 47 | Val 71 | conflict |
His 117 | Ala 141 | engineered mutation |
- | Asn 142 | deletion |
- | Ala 143 | deletion |
Tyr 121 | Lys 145 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 14634.9 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 572.7 | |
All* | Total formula weight | 15207.5 |