6QSQ
X-ray crystal structure of the R336L Vibrio alkaline phosphatase variant.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alkaline phosphatase | polymer | 512 | 56594.1 | 1 | UniProt (A0A2N7KUA1) | Vibrio splendidus | |
| 2 | B (A) | 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID | non-polymer | 214.3 | 1 | Chemie (DTB) | |||
| 3 | C, D (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | F (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | G (A) | water | water | 18.0 | 236 | Chemie (HOH) |
Sequence modifications
A: 1 - 502 (UniProt: A0A2N7KUA1)
| PDB | External Database | Details |
|---|---|---|
| Val 41 | Ile 64 | conflict |
| Ser 79 | Gly 102 | conflict |
| Leu 336 | Arg 359 | engineered mutation |
| Leu 503 | - | expression tag |
| Ala 504 | - | expression tag |
| Trp 505 | - | expression tag |
| Ser 506 | - | expression tag |
| His 507 | - | expression tag |
| Pro 508 | - | expression tag |
| Gln 509 | - | expression tag |
| Phe 510 | - | expression tag |
| Glu 511 | - | expression tag |
| Lys 512 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 56594.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 465.4 | |
| All* | Total formula weight | 57059.5 |
