6QNS
Crystal structure of the binding domain of Botulinum Neurotoxin type B mutant I1248W/V1249W in complex with human synaptotagmin 1 and GD1a receptors
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Botulinum neurotoxin type B | polymer | 442 | 53186.8 | 1 | UniProt (P10844) Pfam (PF07953) Pfam (PF07951) | Clostridium botulinum | BoNT/B,Bontoxilysin-B |
| 2 | B (S) | Synaptotagmin-1 | polymer | 21 | 2471.8 | 1 | UniProt (P21579) | Homo sapiens | Synaptotagmin I,SytI,p65 |
| 3 | C (B) | N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-galactopyranose-(1-4)-[N-acetyl-alpha-neuraminic acid-(2-3)]beta-D-galactopyranose-(1-4)-beta-D-glucopyranose | branched | 1290.1 | 1 | In PDB GlyTouCan (G46677TE) | |||
| 4 | D (A) | water | water | 18.0 | 85 | Chemie (HOH) |
Sequence modifications
A: 857 - 1291 (UniProt: P10844)
| PDB | External Database | Details |
|---|---|---|
| Gly 850 | - | expression tag |
| Ser 851 | - | expression tag |
| His 852 | - | expression tag |
| Met 853 | - | expression tag |
| Ala 854 | - | expression tag |
| Ser 855 | - | expression tag |
| Met 856 | - | expression tag |
| Trp 1248 | Ile 1248 | engineered mutation |
| Trp 1249 | Val 1249 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 55658.6 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1290.1 | |
| All* | Total formula weight | 56948.8 |
