6QE5

Structure of E.coli RlmJ in complex with the natural cofactor product S-adenosyl-homocysteine (SAH)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ribosomal RNA large subunit methyltransferase J	polymer	300	34161.0	2	UniProt (A0A0G3KF30) Pfam (PF04378) UniProt (by SIFTS) (P37634) In PDB	Escherichia coli PCN033	23S rRNA (adenine(2030)-N6)-methyltransferase,23S rRNA m6A2030 methyltransferase
2	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3		water	water		18.0	283	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: A0A0G3KF30)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Lys 60	Glu 60	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68322.1
Non-Polymers*	Number of molecules	2
	Total formula weight	768.8
All*	Total formula weight	69090.9

*Water molecules are not included.