6QDW

Cryo-EM structure of the 50S ribosomal subunit at 2.83 Angstroms with modeled GBC SecM peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (0)	50S ribosomal protein L28	polymer	78	9027.6	1	UniProt (A0A140N3L9) Pfam (PF00830) UniProt (by SIFTS) (P0A7M2)	Escherichia coli (strain B / BL21-DE3)
2	B (1)	50S ribosomal protein L29	polymer	63	7286.5	1	UniProt (A0A140N7F5) Pfam (PF00831) UniProt (by SIFTS) (P0A7M6)	Escherichia coli (strain B / BL21-DE3)
3	C (2)	50S ribosomal protein L30	polymer	59	6554.8	1	UniProt (A0A140N7G4) Pfam (PF00327) UniProt (by SIFTS) (P0AG51)	Escherichia coli (strain B / BL21-DE3)
4	D (3)	50S ribosomal protein L32	polymer	57	6463.4	1	UniProt (A0A140NAZ2) Pfam (PF01783) UniProt (by SIFTS) (P0A7N4)	Escherichia coli (strain B / BL21-DE3)
5	E (4)	50S ribosomal protein L33	polymer	55	6388.6	1	UniProt (A0A140N5Z7) Pfam (PF00471) UniProt (by SIFTS) (P0A7N9)	Escherichia coli (strain B / BL21-DE3)
6	F (6)	50S ribosomal protein L34	polymer	46	5397.5	1	UniProt (A0A140NHV0) Pfam (PF00468) UniProt (by SIFTS) (P0A7P5)	Escherichia coli (strain B / BL21-DE3)
7	G (7)	50S ribosomal protein L35	polymer	65	7313.0	1	UniProt (A0A140N9E3) Pfam (PF01632) UniProt (by SIFTS) (P0A7Q1)	Escherichia coli (strain B / BL21-DE3)
8	H (8)	50S ribosomal protein L36	polymer	38	4377.4	1	UniProt (A0A140N5M6) Pfam (PF00444) UniProt (by SIFTS) (P0A7Q6)	Escherichia coli (strain B / BL21-DE3)
9	I (a)	5S rRNA	polymer	120	38790.1	1	GenBank (1706539273)	Escherichia coli (strain B / BL21-DE3)
10	J (b)	23S rRNA	polymer	2906	942342.9	1	GenBank (296142109)	Escherichia coli BL21(DE3)
11	K (c)	50S ribosomal protein L2	polymer	273	29923.6	1	UniProt (A0A140N7J1) Pfam (PF03947) Pfam (PF00181) UniProt (by SIFTS) (P60422)	Escherichia coli (strain B / BL21-DE3)
12	L (d)	50S ribosomal protein L3	polymer	209	22277.5	1	UniProt (A0A140N3G7) Pfam (PF00297) UniProt (by SIFTS) (P60438)	Escherichia coli (strain B / BL21-DE3)
13	M (e)	50S ribosomal protein L4	polymer	201	22121.6	1	UniProt (A0A140N5K8) Pfam (PF00573) UniProt (by SIFTS) (P60723)	Escherichia coli (strain B / BL21-DE3)
14	N (f)	50S ribosomal protein L5	polymer	179	20333.6	1	UniProt (A0A140N5A3) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (P62399)	Escherichia coli (strain B / BL21-DE3)
15	O (g)	50S ribosomal protein L6	polymer	177	18932.8	1	UniProt (A0A140N2T1) Pfam (PF00347) UniProt (by SIFTS) (P0AG55)	Escherichia coli (strain B / BL21-DE3)
16	P (h)	50S ribosomal protein L9	polymer	149	15789.0	1	UniProt (A0A140NDV1) Pfam (PF01281) Pfam (PF03948) UniProt (by SIFTS) (P0A7R1)	Escherichia coli (strain B / BL21-DE3)
17	Q (j)	50S ribosomal protein L13	polymer	142	16050.6	1	UniProt (A0A140N598) Pfam (PF00572) UniProt (by SIFTS) (P0AA10)	Escherichia coli (strain B / BL21-DE3)
18	R (k)	50S ribosomal protein L14	polymer	123	13565.1	1	UniProt (A0A140N3H4) Pfam (PF00238) UniProt (by SIFTS) (P0ADY3)	Escherichia coli (strain B / BL21-DE3)
19	S (l)	50S ribosomal protein L15	polymer	144	14994.4	1	UniProt (A0A140N711) Pfam (PF00828) UniProt (by SIFTS) (P02413)	Escherichia coli (strain B / BL21-DE3)
20	T (m)	50S ribosomal protein L16	polymer	136	15312.3	1	UniProt (A0A140N6Z2) Pfam (PF00252) UniProt (by SIFTS) (P0ADY7)	Escherichia coli (strain B / BL21-DE3)
21	U (n)	50S ribosomal protein L17	polymer	127	14393.7	1	UniProt (A0A140N4M0) Pfam (PF01196) UniProt (by SIFTS) (P0AG44)	Escherichia coli (strain B / BL21-DE3)
22	V (o)	50S ribosomal protein L18	polymer	117	12794.7	1	UniProt (A0A140N4L0) Pfam (PF00861) UniProt (by SIFTS) (P0C018)	Escherichia coli (strain B / BL21-DE3)
23	W (p)	50S ribosomal protein L19	polymer	115	13159.3	1	UniProt (A0A140N6T7) Pfam (PF01245) UniProt (by SIFTS) (P0A7K6)	Escherichia coli (strain B / BL21-DE3)
24	X (q)	50S ribosomal protein L20	polymer	118	13528.0	1	UniProt (A0A140NBS1) Pfam (PF00453) UniProt (by SIFTS) (P0A7L3)	Escherichia coli (strain B / BL21-DE3)
25	Y (r)	50S ribosomal protein L21	polymer	103	11586.4	1	UniProt (A0A140N5D7) Pfam (PF00829) UniProt (by SIFTS) (P0AG48)	Escherichia coli (strain B / BL21-DE3)
26	Z (s)	50S ribosomal protein L22	polymer	110	12253.4	1	UniProt (A0A140N2S3) Pfam (PF00237) UniProt (by SIFTS) (P61175)	Escherichia coli (strain B / BL21-DE3)
27	AA (t)	50S ribosomal protein L23	polymer	100	11222.2	1	UniProt (C6EGE8) Pfam (PF00276)	Escherichia coli (strain B / BL21-DE3)
28	BA (u)	50S ribosomal protein L24	polymer	104	11339.3	1	UniProt (A0A140N5L7) Pfam (PF00467) Pfam (PF17136) UniProt (by SIFTS) (P60624)	Escherichia coli (strain B / BL21-DE3)
29	CA (v)	glycine-tRNA glyT	polymer	75	23935.1	1	GenBank (1730024484)	Escherichia coli (strain B / BL21-DE3)
30	DA (w)	50S ribosomal protein L25	polymer	94	10713.5	1	UniProt (A0A140N846) Pfam (PF01386) UniProt (by SIFTS) (P68919)	Escherichia coli (strain B / BL21-DE3)
31	EA (y)	50S ribosomal protein L27	polymer	85	9146.5	1	UniProt (A0A140N340) Pfam (PF01016) UniProt (by SIFTS) (P0A7L8)	Escherichia coli (strain B / BL21-DE3)
32	FA (z)	Gamma-crystallin B	polymer	61	7158.0	1	UniProt (P02526) Pfam (PF00030)	Bos taurus (Bovine)	Gamma-B-crystallin,Gamma-crystallin II

Sequence modifications

z: -20 - 17 (UniProt: P02526)

PDB	External Database	Details
Met -32	-	initiating methionine
Gly -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Phe 13	Asn 34	conflict
Thr 15	Ile 36	conflict
Pro 16	Arg 37	conflict
Trp 18	-	expression tag
Ile 19	-	expression tag
Ser 20	-	expression tag
Gln 21	-	expression tag
Ala 22	-	expression tag
Gln 23	-	expression tag
Gly 24	-	expression tag
Ile 25	-	expression tag
Arg 26	-	expression tag
Ala 27	-	expression tag
Gly 28	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	32
	Total formula weight	1374472.2
All*	Total formula weight	1374472.2

*Water molecules are not included.