6Q52

Structure of a psychrophilic CCA-adding enzyme in complex with CMPcPP at room temperature in ChipX microfluidic device

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CCA-adding enzyme	polymer	420	48498.3	1	UniProt (A0A1C7DQ98)	Planococcus halocryophilus
2	B (A)	5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine	non-polymer		481.2	1	Chemie (2TM)
3	C (A)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

A: 1 - 377 (UniProt: A0A1C7DQ98)

PDB	External Database	Details
Met -42	-	initiating methionine
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
His -38	-	expression tag
His -37	-	expression tag
His -36	-	expression tag
Ser -35	-	expression tag
Ser -34	-	expression tag
Gly -33	-	expression tag
Leu -32	-	expression tag
Val -31	-	expression tag
Pro -30	-	expression tag
Arg -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Gly -26	-	expression tag
Met -25	-	expression tag
Lys -24	-	expression tag
Glu -23	-	expression tag
Thr -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Ala -19	-	expression tag
Lys -18	-	expression tag
Phe -17	-	expression tag
Glu -16	-	expression tag
Arg -15	-	expression tag
Gln -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Asp -11	-	expression tag
Ser -10	-	expression tag
Pro -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Gly -6	-	expression tag
Thr -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	48498.3
Non-Polymers*	Number of molecules	1
	Total formula weight	481.2
All*	Total formula weight	48979.5

*Water molecules are not included.