6Q4Z

Structure of an inactive variant (D94N) of MPT-2, a GDP-Man-dependent mannosyltransferase from Leishmania mexicana, in complex with beta-1,2-mannobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LmxM MPT-2 D94N	polymer	341	38092.3	2	UniProt (E9AND8) Pfam (PF08950)	Leishmania mexicana MHOM/GT/2001/U1103
2	C, D (C, D)	beta-D-mannopyranose-(1-2)-alpha-D-mannopyranose	branched		342.3	2	In PDB GlyTouCan (G08254PX)
3	E, F (A, B)	water	water		18.0	479	Chemie (HOH)

Sequence modifications

A, B: 1 - 321 (UniProt: E9AND8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 94	Asp 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76184.5
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	76869.1

*Water molecules are not included.