6Q2O

Cryo-EM structure of RET/GFRa2/NRTN extracellular complex. The 3D refinement was applied with C2 symmetry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Neurturin	polymer	102	11706.4	2	UniProt (Q99748) Pfam (PF00019)	Homo sapiens (Human)
2	C, E (C, D)	GDNF family receptor alpha-2	polymer	349	39572.6	2	UniProt (O00451) Pfam (PF02351)	Homo sapiens (Human)	GFR-alpha-2,GDNF receptor beta,GDNFR-beta,Neurturin receptor alpha,NTNR-alpha,RET ligand 2,TGF-beta-related neurotrophic factor receptor 2
3	D, F (E, F)	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	617	69100.8	2	UniProt (P07949) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813) Pfam (PF22540)	Homo sapiens (Human)	Cadherin family member 12,Proto-oncogene c-Ret
4	G, H, I, J, Q... (E, F)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
5	K, L, M, N, O... (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG)

Sequence modifications

C, D: 24 - 362 (UniProt: O00451)

PDB	External Database	Details
Gly 363	-	expression tag
Thr 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag

E, F: 29 - 635 (UniProt: P07949)

PDB	External Database	Details
His 114	Arg 114	conflict
Gly 636	-	expression tag
Thr 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	240759.7
Non-Polymers*	Number of molecules	20
	Total formula weight	2975.1
All*	Total formula weight	243734.8

*Water molecules are not included.