6PXI

The crystal structure of a singly capped HslUV complex with an axial pore plug and a HslU E257Q mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	ATP-dependent protease subunit HslV	polymer	174	18915.6	4	UniProt (P0A7B8) Pfam (PF00227) In PDB	Escherichia coli	Heat shock protein HslV
2	E, F	ATP-dependent protease ATPase subunit HslU	polymer	448	50356.4	2	UniProt (P0A6H5) Pfam (PF00004) Pfam (PF07724) In PDB	Escherichia coli	Heat shock protein HslU,Unfoldase HslU
3	A, C	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	E, F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
5		water	water		18.0	1	Chemie (HOH)

Sequence modifications

E, F: 2 - 443 (UniProt: P0A6H5)

PDB	External Database	Details
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 257	Glu 257	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	176375.1
Non-Polymers*	Number of molecules	5
	Total formula weight	1142.6
All*	Total formula weight	177517.6

*Water molecules are not included.