6PWX

Cryo-EM structure of RbBP5 bound to the nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Retinoblastoma-binding protein 5	polymer	538	59179.4	1	UniProt (Q15291) Pfam (PF00400)	Homo sapiens (Human)	RBBP-5,Retinoblastoma-binding protein RBQ-3
2	B, F (G, K)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
3	C, K (H, L)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
4	D, G (I, M)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
5	E, H (J, N)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
6	I (O)	DNA (146-MER)	polymer	147	45138.8	1		synthetic construct
7	J (P)	DNA (146-MER)	polymer	147	45610.0	1		synthetic construct

Sequence modifications

A: 2 - 538 (UniProt: Q15291)

PDB	External Database	Details
Ser 1	-	expression tag

G, K: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

I, M: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

J, N: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	258855.7
All*	Total formula weight	258855.7

*Water molecules are not included.