6PWV

Cryo-EM structure of MLL1 core complex bound to the nucleosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Retinoblastoma-binding protein 5	polymer	538	59179.4	1	UniProt (Q15291) Pfam (PF00400) In PDB	Homo sapiens (Human)	RBBP-5,Retinoblastoma-binding protein RBQ-3
2	B	WD repeat-containing protein 5	polymer	313	34391.0	1	UniProt (P61964) Pfam (PF00400) In PDB	Homo sapiens (Human)	BMP2-induced 3-kb gene protein
3	C	Histone-lysine N-methyltransferase 2A	polymer	209	24141.7	1	UniProt (Q03164) Pfam (PF00856) In PDB	Homo sapiens (Human)	Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX
4	G, K	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	Histone H3
5	H, L	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
6	I, M	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
7	J, N	Histone H2B 1.1	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
8	O	DNA (147-MER)	polymer	147	45138.8	1		synthetic construct
9	P	DNA (147-MER)	polymer	147	45610.0	1		synthetic construct
10	E, F	Protein dpy-30 homolog	polymer	102	11492.9	2	UniProt (Q9C005) Pfam (PF05186) In PDB	Homo sapiens (Human)	Dpy-30-like protein,Dpy-30L
11	D	Set1/Ash2 histone methyltransferase complex subunit ASH2	polymer	534	60244.6	1	UniProt (Q9UBL3) Pfam (PF21257) Pfam (PF21198) Pfam (PF00622) In PDB	Homo sapiens (Human)	ASH2-like protein
12	C	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
13	C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 2 - 538 (UniProt: Q15291)

PDB	External Database	Details
Ser 1	-	expression tag

C: 3762 - 3969 (UniProt: Q03164)

PDB	External Database	Details
Ser 3761	-	expression tag
Ile 3861	Asn 3861	conflict
Leu 3867	Gln 3867	conflict

G, K: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

I, M: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

J, N: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	conflict

E, F: 1 - 99 (UniProt: Q9C005)

PDB	External Database	Details
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D: 2 - 534 (UniProt: Q9UBL3)

PDB	External Database	Details
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	400356.4
Non-Polymers*	Number of molecules	4
	Total formula weight	899.6
All*	Total formula weight	401256.0

*Water molecules are not included.