6PWF

Cryo-EM structure of the ATPase domain of chromatin remodeling factor ISWI bound to the nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	136	15421.1	2	UniProt (P02299) Pfam (PF00125)	Drosophila melanogaster (Fruit fly)
2	B, F (B, F)	Histone H4	polymer	103	11408.5	2	UniProt (A0A0B4KFZ9) Pfam (PF15511) UniProt (by SIFTS) (P84040)	Drosophila melanogaster (Fruit fly)
3	C, G (C, G)	Histone H2A	polymer	124	13388.7	2	UniProt (P84051) Pfam (PF00125) Pfam (PF16211)	Drosophila melanogaster (Fruit fly)
4	D, H (D, H)	Histone H2B	polymer	123	13727.1	2	UniProt (P02283) Pfam (PF00125)	Drosophila melanogaster (Fruit fly)
5	I (I)	DNA (147-MER)	polymer	147	45610.0	1		synthetic construct
6	J (J)	DNA (147-MER)	polymer	147	45138.8	1		synthetic construct
7	K (K)	chromatin remodeling factor ISWI	polymer	640	73458.5	1	UniProt (G0S9L5) Pfam (PF00176) Pfam (PF00271)	Chaetomium thermophilum	Complex ATPase-like protein

Sequence modifications

K: 105 - 162 (UniProt: G0S9L5)

PDB	External Database	Details
Met 83	-	expression tag
Ala 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
Gly 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag
His 94	-	expression tag
Glu 95	-	expression tag
Asn 96	-	expression tag
Leu 97	-	expression tag
Tyr 98	-	expression tag
Phe 99	-	expression tag
Gln 100	-	expression tag
Gly 101	-	expression tag
Ser 102	-	expression tag
Ser 103	-	expression tag
Ser 104	-	expression tag

K: 167 - 722 (UniProt: G0S9L5)

PDB	External Database	Details
Gly 163	-	linker
Ser 164	-	linker
Ser 165	-	linker
Gly 166	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	272098.0
All*	Total formula weight	272098.0

*Water molecules are not included.