6PT0
Cryo-EM structure of human cannabinoid receptor 2-Gi protein in complex with agonist WIN 55,212-2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Cannabinoid receptor 2 | polymer | 369 | 40764.8 | 1 | UniProt (P34972) Pfam (PF00001) | Homo sapiens (Human) | hCB2,CX5 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 354 | 39151.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | D (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 259 | 27784.9 | 1 | synthetic construct | ||
| 6 | F (R) | {(3R)-5-methyl-3-[(morpholin-4-yl)methyl]-2,3-dihydro[1,4]oxazino[2,3,4-hi]indol-6-yl}(naphthalen-1-yl)methanone | non-polymer | 426.5 | 1 | Chemie (WI5) | |||
| 7 | G, L, M, N (R) | PALMITIC ACID | non-polymer | 256.4 | 4 | Chemie (PLM) | |||
| 8 | H, I, J, K (R) | CHOLESTEROL | non-polymer | 386.7 | 4 | Chemie (CLR) |
Sequence modifications
R: 1 - 360 (UniProt: P34972)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Ala 361 | - | expression tag |
| Ala 362 | - | expression tag |
| Ala 363 | - | expression tag |
| His 364 | - | expression tag |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| Met -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 155679.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2998.8 | |
| All* | Total formula weight | 158678.2 |
