6PT0

Cryo-EM structure of human cannabinoid receptor 2-Gi protein in complex with agonist WIN 55,212-2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Cannabinoid receptor 2	polymer	369	40764.8	1	UniProt (P34972) Pfam (PF00001) In PDB	Homo sapiens (Human)	hCB2,CX5
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39151.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	E	scFv16	polymer	259	27784.9	1		synthetic construct
6	R	{(3R)-5-methyl-3-[(morpholin-4-yl)methyl]-2,3-dihydro[1,4]oxazino[2,3,4-hi]indol-6-yl}(naphthalen-1-yl)methanone	non-polymer		426.5	1	Chemie (WI5)
7	R	PALMITIC ACID	non-polymer		256.4	4	Chemie (PLM)
8	R	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR)

Sequence modifications

R: 1 - 360 (UniProt: P34972)

PDB	External Database	Details
Ala 361	-	expression tag
Ala 362	-	expression tag
Ala 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	155679.4
Non-Polymers*	Number of molecules	9
	Total formula weight	2998.8
All*	Total formula weight	158678.2

*Water molecules are not included.