6PSV

Escherichia coli RNA polymerase promoter unwinding intermediate (TpreRPo) with TraR and rpsT P2 promoter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	N	Protein TraR	polymer	72	8193.3	1	UniProt (P41065) Pfam (PF01258) In PDB	Escherichia coli	RNA polymerase transcription factor TraR
2	G, H, M	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	3	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
3	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4	J	DNA-directed RNA polymerase subunit beta'	polymer	1430	158073.6	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6	L	RNA polymerase sigma factor RpoD	polymer	616	70715.6	1	UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) In PDB	Escherichia coli	Sigma-70
7	O	DNA (85-MER)	polymer	85	26235.8	1		Escherichia coli
8	P	DNA (85-MER)	polymer	85	26190.8	1		Escherichia coli
9	N, J	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
10	N, I, J, L	CHAPSO	non-polymer		631.9	4	Chemie (1N7)
11	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

J: 2 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag
Gly 1416	-	expression tag
Pro 1417	-	expression tag
Ser 1418	-	expression tag
Ser 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

L: 1 - 613 (UniProt: Q0P6L9)

PDB	External Database	Details
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	560155.6
Non-Polymers*	Number of molecules	8
	Total formula weight	2748.1
All*	Total formula weight	562903.6

*Water molecules are not included.