6PSU
Escherichia coli RNA polymerase promoter unwinding intermediate (TRPi2) with TraR and rpsT P2 promoter
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (N) | Protein TraR | polymer | 72 | 8193.3 | 1 | UniProt (P41065) Pfam (PF01258) | Escherichia coli | RNA polymerase transcription factor TraR |
| 2 | B, C, J (G, H, M) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 3 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 3 | D (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | E (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1430 | 158073.6 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | F (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 6 | G (L) | RNA polymerase sigma factor RpoD | polymer | 616 | 70715.6 | 1 | UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) | Escherichia coli | Sigma-70 |
| 7 | H (O) | DNA (85-MER) | polymer | 85 | 26235.8 | 1 | Escherichia coli | ||
| 8 | I (P) | DNA (85-MER) | polymer | 85 | 26190.8 | 1 | Escherichia coli | ||
| 9 | K, O, P (N, J) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 10 | L, M, Q, R (N, I, J, L) | CHAPSO | non-polymer | 631.9 | 4 | Chemie (1N7) | |||
| 11 | N (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
J: 2 - 1407 (UniProt: P0A8T7)
L: 1 - 613 (UniProt: Q0P6L9)
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
| Leu 1408 | - | expression tag |
| Glu 1409 | - | expression tag |
| Leu 1410 | - | expression tag |
| Glu 1411 | - | expression tag |
| Val 1412 | - | expression tag |
| Leu 1413 | - | expression tag |
| Phe 1414 | - | expression tag |
| Gln 1415 | - | expression tag |
| Gly 1416 | - | expression tag |
| Pro 1417 | - | expression tag |
| Ser 1418 | - | expression tag |
| Ser 1419 | - | expression tag |
| Gly 1420 | - | expression tag |
| His 1421 | - | expression tag |
| His 1422 | - | expression tag |
| His 1423 | - | expression tag |
| His 1424 | - | expression tag |
| His 1425 | - | expression tag |
| His 1426 | - | expression tag |
| His 1427 | - | expression tag |
| His 1428 | - | expression tag |
| His 1429 | - | expression tag |
| His 1430 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 560155.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2748.1 | |
| All* | Total formula weight | 562903.6 |
