6PQU

Cryo-EM structure of HzTransib/nicked TIR substrate DNA pre-reaction complex (PRC)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	DNA-mediated transposase	polymer	497	56582.7	2	UniProt (B0F0C5)	Helicoverpa zea (Corn earworm moth)
2	B, F (B, F)	DNA (5'-D(P*AP*TP*CP*TP*GP*GP*CP*CP*TP*AP*GP*AP*TP*CP*T)-3')	polymer	16	4889.2	2		Helicoverpa zea
3	C, G (C, G)	DNA (5'-D(P*CP*AP*CP*GP*GP*TP*GP*GP*AP*TP*CP*GP*AP*AP*AP*A)-3')	polymer	16	4956.2	2		Helicoverpa zea
4	D, H (D, H)	TIR substrate DNA transferred strand	polymer	32	9792.3	2		Helicoverpa zea
5	I, L (A, E)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	J, M (A, E)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	K, N (A, E)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)

Sequence modifications

A, E: 17 - 507 (UniProt: B0F0C5)

PDB	External Database	Details
His 508	-	expression tag
His 509	-	expression tag
His 510	-	expression tag
His 511	-	expression tag
His 512	-	expression tag
His 513	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	152440.9
Non-Polymers*	Number of molecules	6
	Total formula weight	257.6
All*	Total formula weight	152698.5

*Water molecules are not included.