6PMI
Sigm28-transcription initiation complex with specific promoter at the state 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB | Escherichia coli O157:H7 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB | Escherichia coli O45:K1 (strain S88 / ExPEC) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB | Escherichia coli O157:H7 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB | Escherichia coli O45:K1 (strain S88 / ExPEC) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F | RNA polymerase sigma factor FliA | polymer | 247 | 28627.3 | 1 | UniProt (P0AEM6) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli (strain K12) | RNA polymerase sigma factor for flagellar operon,Sigma F,Sigma-27,Sigma-28 |
| 6 | 1 | SYNTHETIC NONTEMPLATE STRAND DNA (54-MER) | polymer | 54 | 16563.6 | 1 | Escherichia coli | ||
| 7 | 2 | SYNTHETIC TEMPLATE STRAND DNA (54-MER) | polymer | 54 | 16759.8 | 1 | Escherichia coli | ||
| 8 | 3 | Nascent RNA | polymer | 3 | 1078.6 | 1 | Escherichia coli | ||
| 9 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
F: 1 - 239 (UniProt: P0AEM6)
| PDB | External Database | Details |
|---|---|---|
| Leu 240 | - | expression tag |
| Glu 241 | - | expression tag |
| His 242 | - | expression tag |
| His 243 | - | expression tag |
| His 244 | - | expression tag |
| His 245 | - | expression tag |
| His 246 | - | expression tag |
| His 247 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 452583.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 452738.9 |
