6PLP

CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA, desensitized state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Glycine receptor subunit alphaZ1	polymer	458	52537.6	5	UniProt (O93430) Pfam (PF02931) Pfam (PF02932) In PDB	Danio rerio (Zebrafish)
2	F, G, H, I, J	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	5	In PDB GlyTouCan (G15407YE)
3	A, B, C, D, E	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	5	Chemie (ABU)

Sequence modifications

A, B, C, D, E: -7 - 436 (UniProt: O93430)

PDB	External Database	Details
Tyr 175	Phe 183	engineered mutation
Phe 177	Tyr 185	engineered mutation
Leu 437	-	expression tag
Val 438	-	expression tag
Pro 439	-	expression tag
Arg 440	-	expression tag
Gly 441	-	expression tag
Ser 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	262688.0
Branched	Number of molecules	5
	Total formula weight	2932.7
Non-Polymers*	Number of molecules	5
	Total formula weight	515.6
All*	Total formula weight	266136.3

*Water molecules are not included.