6PJR

Time-resolved structural snapshot of proteolysis by GlpG inside the membrane

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Rhomboid protease GlpG	polymer	211	23800.1	1	UniProt (A0A0J2E248) Pfam (PF01694) UniProt (by SIFTS) (P09391)	Escherichia coli	Intramembrane serine protease
2	B (B)	Peptide aldehyde inhibitor	polymer	13	1524.9	1	UniProt (by SIFTS) (P42287)	Drosophila melanogaster
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D, E (A, B)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A: 87 - 276 (UniProt: A0A0J2E248)

PDB	External Database	Details
Met 66	-	initiating methionine
Gly 67	-	expression tag
Ser 68	-	expression tag
Ser 69	-	expression tag
His 70	-	expression tag
His 71	-	expression tag
His 72	-	expression tag
His 73	-	expression tag
His 74	-	expression tag
His 75	-	expression tag
Ser 76	-	expression tag
Ser 77	-	expression tag
Gly 78	-	expression tag
Leu 79	-	expression tag
Val 80	-	expression tag
Pro 81	-	expression tag
Arg 82	-	expression tag
Gly 83	-	expression tag
Ser 84	-	expression tag
His 85	-	expression tag
Met 86	-	expression tag
Phe 205	Tyr 205	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25325.0
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	25360.5

*Water molecules are not included.