6PB5

The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	1	UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor RpoD	polymer	628	72206.3	1	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli	Sigma-70
6	G, H	cAMP-activated global transcriptional regulator CRP	polymer	210	23672.4	2	UniProt (P0ACK0) Pfam (PF00027) Pfam (PF13545) UniProt (by SIFTS) (P0ACJ8) In PDB	Escherichia coli	Catabolite activator protein,CAP,Catabolite gene activator,cAMP receptor protein,CRP,cAMP regulatory protein
7	1	SYNTHETIC NONTEMPLATE STRAND DNA (78-MER)	polymer	78	23976.3	1		Escherichia coli
8	2	SYNTHETIC TEMPLATE STRAND DNA (78-MER)	polymer	78	24191.6	1		Escherichia coli
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	G, H	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE	non-polymer		329.2	2	Chemie (CMP)

Sequence modifications

F: 1 - 613 (UniProt: P00579)

PDB	External Database	Details
Met -14	-	expression tag
Arg -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Thr -4	-	expression tag
Asp -3	-	expression tag
Gln -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	557273.6
Non-Polymers*	Number of molecules	5
	Total formula weight	813.5
All*	Total formula weight	558087.2

*Water molecules are not included.