6PB5
The E. coli class-II CAP-dependent transcription activation complex at the state 1 architecture
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (B7MIX3) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T8) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (B7MFL0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 628 | 72206.3 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli | Sigma-70 |
| 6 | G, H (G, H) | cAMP-activated global transcriptional regulator CRP | polymer | 210 | 23672.4 | 2 | UniProt (P0ACK0) Pfam (PF00027) Pfam (PF13545) UniProt (by SIFTS) (P0ACJ8) | Escherichia coli | Catabolite activator protein,CAP,Catabolite gene activator,cAMP receptor protein,CRP,cAMP regulatory protein |
| 7 | I (1) | SYNTHETIC NONTEMPLATE STRAND DNA (78-MER) | polymer | 78 | 23976.3 | 1 | Escherichia coli | ||
| 8 | J (2) | SYNTHETIC TEMPLATE STRAND DNA (78-MER) | polymer | 78 | 24191.6 | 1 | Escherichia coli | ||
| 9 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 11 | N, O (G, H) | ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE | non-polymer | 329.2 | 2 | Chemie (CMP) PubChem (6076) PubChem (216878) PubChem (3080770) PubChem (3246347) PubChem (6603718) PubChem (6713792) PubChem (6714006) PubChem (12071879) PubChem (12866365) PubChem (23902375) PubChem (25322948) PubChem (40467852) PubChem (44629928) PubChem (176488666) PubChem (25322946) PubChem (59350541) PubChem (274) PubChem (6604194) PubChem (56973690) PubChem (163043517) PubChem (59909322) PubChem (59968988) PubChem (70878457) PubChem (124132380) PubChem (129317551) PubChem (131841987) PubChem (134693155) PubChem (138107790) PubChem (163043518) |
Sequence modifications
F: 1 - 613 (UniProt: P00579)
| PDB | External Database | Details |
|---|---|---|
| Met -14 | - | expression tag |
| Arg -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| Thr -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 557273.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 813.5 | |
| All* | Total formula weight | 558087.2 |
