6PAK
Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Subtilisin E | polymer | 285 | 29136.2 | 2 | UniProt (P04189) Pfam (PF00082) In PDB | Bacillus subtilis (strain 168) | |
2 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
3 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
4 | water | water | 18.0 | 454 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 276 (UniProt: P04189)
PDB | External Database | Details |
---|---|---|
Ser 130 | Thr 236 | engineered mutation |
Gln 158 | Ser 264 | engineered mutation |
His 159 | Ser 265 | engineered mutation |
Pro 160 | Gly 266 | engineered mutation |
Lys 161 | Ser 267 | engineered mutation |
Glu 162 | Thr 268 | engineered mutation |
Gly 163 | - | insertion |
Cys 222 | Ser 327 | engineered mutation |
Ser 277 | - | expression tag |
Leu 278 | - | expression tag |
Glu 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
His 282 | - | expression tag |
His 283 | - | expression tag |
His 284 | - | expression tag |
His 285 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 58272.3 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 328.4 | |
All* | Total formula weight | 58600.8 |