6PAA

E. coli L-asparaginase II mutant (T12V) in complex with L-Asp at pH 5.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-asparaginase 2	polymer	334	35700.0	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, B, C, D	ASPARTIC ACID	non-polymer		133.1	4	Chemie (ASP)
3		water	water		18.0	1075	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 12	Thr 34	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142800.0
Non-Polymers*	Number of molecules	4
	Total formula weight	532.4
All*	Total formula weight	143332.4

*Water molecules are not included.