6PA9

E. coli L-asparaginase II mutant (T12V) in complex with L-Asn at pH 7.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	L-asparaginase 2	polymer	334	35700.0	2	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	C, D (A, B)	ASPARAGINE	non-polymer		132.1	2	Chemie (ASN)
3	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G (A, B)	water	water		18.0	486	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 12	Thr 34	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	71400.0
Non-Polymers*	Number of molecules	3
	Total formula weight	356.3
All*	Total formula weight	71756.3

*Water molecules are not included.