6PA7

The cryo-EM structure of the human DNMT3A2-DNMT3B3 complex bound to nucleosome.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (167-MER)	polymer	167	51327.7	1		synthetic construct
6	J (J)	DNA (167-MER)	polymer	167	51786.0	1		synthetic construct
7	K, M (K, P)	DNA (cytosine-5)-methyltransferase 3A	polymer	689	77914.7	2	UniProt (Q9Y6K1) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145)	Homo sapiens (Human)	Dnmt3a,DNA methyltransferase HsaIIIA,M.HsaIIIA
8	L, N (N, S)	DNA (cytosine-5)-methyltransferase 3B	polymer	770	86294.9	2	UniProt (Q9UBC3) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145)	Homo sapiens (Human)	Dnmt3b,DNA methyltransferase HsaIIIB,M.HsaIIIB
9	O, P, Q (A, C, H)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
10	R, S (K, P)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	conflict

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	540198.0
Non-Polymers*	Number of molecules	5
	Total formula weight	875.2
All*	Total formula weight	541073.2

*Water molecules are not included.