6PA5

ECAII(T89V,K162T) MUTANT IN COMPLEX WITH L-ASN AT PH 8.3 IN SPACE GROUP P2(1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	L-asparaginase 2	polymer	334	35671.9	8	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, B, C, D, E...	ASPARAGINE	non-polymer		132.1	8	Chemie (ASN)
3	A, B, C, D, E...	IMIDAZOLE	non-polymer		69.1	7	Chemie (IMD)
4	A, B, D	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5		water	water		18.0	2107	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 89	Thr 111	engineered mutation
Thr 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	285375.3
Non-Polymers*	Number of molecules	18
	Total formula weight	1816.8
All*	Total formula weight	287192.1

*Water molecules are not included.