6PA5
ECAII(T89V,K162T) MUTANT IN COMPLEX WITH L-ASN AT PH 8.3 IN SPACE GROUP P2(1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | L-asparaginase 2 | polymer | 334 | 35671.9 | 8 | UniProt (P00805) Pfam (PF00710) Pfam (PF17763) | Escherichia coli (strain K12) | L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II |
| 2 | I, L, O, Q, T... (A, B, C, D, E...) | ASPARAGINE | non-polymer | 132.1 | 8 | Chemie (ASN) | |||
| 3 | J, M, P, R, U... (A, B, C, D, E...) | IMIDAZOLE | non-polymer | 69.1 | 7 | Chemie (IMD) | |||
| 4 | K, N, S (A, B, D) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | AA, BA, CA, DA, EA... (A, B, C, D, E...) | water | water | 18.0 | 2107 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 326 (UniProt: P00805)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Asp -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Val 89 | Thr 111 | engineered mutation |
| Thr 162 | Lys 184 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 285375.3 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 1816.8 | |
| All* | Total formula weight | 287192.1 |
