6PA3

E. coli L-asparaginase II double mutant (T89V,K162T) in complex with L-Asn at pH 7.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	334	35671.9	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	E, J, K, L (A, B, C, D)	ASPARAGINE	non-polymer		132.1	4	Chemie (ASN)
3	F, G, H (A)	IMIDAZOLE	non-polymer		69.1	3	Chemie (IMD)
4	I (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	M, N, O, P (A, B, C, D)	water	water		18.0	1175	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Val 89	Thr 111	engineered mutation
Thr 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142687.7
Non-Polymers*	Number of molecules	8
	Total formula weight	760.0
All*	Total formula weight	143447.7

*Water molecules are not included.