6PA2

E. coli L-asparaginase II mutant (K162M) in complex with L-Asp at pH 5.6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, B, D	L-asparaginase 2	polymer	334	35704.0	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763) In PDB	Escherichia coli (strain K12)	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	A, C, B, D	ASPARTIC ACID	non-polymer		133.1	4	Chemie (ASP)
3	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	1170	Chemie (HOH)

Sequence modifications

A, C, B, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Met 162	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142815.9
Non-Polymers*	Number of molecules	5
	Total formula weight	624.5
All*	Total formula weight	143440.4

*Water molecules are not included.