6P7N
Cryo-EM structure of LbCas12a-crRNA: AcrVA4 (2:2 complex)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (C, G) | anti-CRISPR VA4 | polymer | 237 | 27641.4 | 2 | UniProt (A0A0U2APF4) | Moraxella bovoculi | AcrVA4 |
| 2 | C, D (A, E) | Cas12a | polymer | 1231 | 144160.6 | 2 | Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) UniProt (by SIFTS) (A0A182DWE3) | Lachnospiraceae bacterium ND2006 | |
| 3 | E, F (B, F) | mature crRNA | polymer | 40 | 12815.6 | 2 | Lachnospiraceae bacterium ND2006 | ||
| 4 | G, H, I, J (A, E, B, F) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
C, G: 1 - 234 (UniProt: A0A0U2APF4)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 369235.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 369332.5 |
