6P19

Q21 transcription antitermination complex: loaded complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	DNA (123-MER) fragment carrying phage-21 pR' promoter, pause element, and transcribed region, nontemplate strand	polymer	123	38545.6	1		Phage 21
2	2	DNA (123-MER) fragment carrying phage-21 pR' promoter, pause element, and transcribed region, template strand	polymer	123	37358.9	1		Phage 21
3	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	D	DNA-directed RNA polymerase subunit beta'	polymer	1430	158314.9	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
7	Q	Q protein	polymer	162	18668.8	1	UniProt (Q9XJQ6) Pfam (PF06530) In PDB	Phage 21	Transcription antitermination factor Q21
8	R	Transcribed RNA	polymer	70	22972.6	1		Escherichia coli	transcribed RNA
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Arg 1410	-	expression tag
Arg 1411	-	expression tag
Ala 1412	-	expression tag
Ser 1413	-	expression tag
Glu 1414	-	expression tag
Asn 1415	-	expression tag
Leu 1416	-	expression tag
Tyr 1417	-	expression tag
Phe 1418	-	expression tag
Gln 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

Q: 2 - 162 (UniProt: Q9XJQ6)

PDB	External Database	Details
Ser 1	-	expression tag
Gly 24	-	insertion
Trp 26	His 25	conflict
Val 27	Gly 26	conflict
Tyr 28	Leu 27	conflict
Val 47	Ile 46	conflict

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	510048.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	510203.7

*Water molecules are not included.