6P18

Q21 transcription antitermination complex: loading complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	DNA (67-MER) fragment carrying phage-21 pR' promoter and pause element, nontemplate strand	polymer	67	20704.3	1		Phage 21
2	2	DNA (67-MER) fragment carrying phage-21 pR' promoter and pause element, template strand	polymer	67	20588.2	1		Phage 21
3	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	D	DNA-directed RNA polymerase subunit beta'	polymer	1430	158314.9	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
7	F	RNA polymerase sigma factor RpoD	polymer	627	71842.8	1	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma-70
8	P, Q	Q protein	polymer	162	18668.8	2	UniProt (Q9XJQ6) Pfam (PF06530) In PDB	Phage 21
9	R	RNA (5'-R(P*UP*GP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')	polymer	11	3626.2	1		Escherichia coli
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Arg 1410	-	expression tag
Arg 1411	-	expression tag
Ala 1412	-	expression tag
Ser 1413	-	expression tag
Glu 1414	-	expression tag
Asn 1415	-	expression tag
Leu 1416	-	expression tag
Tyr 1417	-	expression tag
Phe 1418	-	expression tag
Gln 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

F: 1 - 613 (UniProt: P00579)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Cys 541	Arg 541	engineered mutation
Pro 607	Leu 607	engineered mutation

P, Q: 2 - 162 (UniProt: Q9XJQ6)

PDB	External Database	Details
Ser 1	-	expression tag
Gly 24	-	insertion
Trp 26	His 25	conflict
Val 27	Gly 26	conflict
Tyr 28	Leu 27	conflict
Val 47	Ile 46	conflict

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	546601.8
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	546756.9

*Water molecules are not included.