6P04

Binary structure of the E52D mutant of ANT-4'' with Neomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Kanamycin nucleotidyltransferase	polymer	256	29121.9	2	UniProt (P05058) Pfam (PF07827) In PDB	Bacillus sp.
2	A	NEOMYCIN	non-polymer		614.6	2	Chemie (NMY)
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4		water	water		18.0	143	Chemie (HOH)

Sequence modifications

A, B: 1 - 253 (UniProt: P05058)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 52	Glu 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58243.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1253.6
All*	Total formula weight	59497.3

*Water molecules are not included.