6OXN
2.6 Angstrom structure of W45F/H46S Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with glyoxylate and NADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glyoxylate/hydroxypyruvate reductase A | polymer | 318 | 36096.1 | 1 | UniProt (A0A140NAE3) Pfam (PF02826) UniProt (by SIFTS) (P75913) | Escherichia coli (strain B / BL21-DE3) | 2-ketoacid reductase |
| 2 | B (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 3 | C (A) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 1 | Chemie (NAP) | |||
| 4 | D (A) | GLYOXYLIC ACID | non-polymer | 74.0 | 1 | Chemie (GLV) | |||
| 5 | E, F, G (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 6 | H (A) | water | water | 18.0 | 118 | Chemie (HOH) |
Sequence modifications
A: 1 - 312 (UniProt: A0A140NAE3)
| PDB | External Database | Details |
|---|---|---|
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Phe 45 | Trp 45 | engineered mutation |
| Ser 46 | His 46 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 36096.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 978.5 | |
| All* | Total formula weight | 37074.6 |
