6OXN

2.6 Angstrom structure of W45F/H46S Glyoxylate/Hydroxypyruvate reductase A from Escherichia Coli in complex with glyoxylate and NADP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glyoxylate/hydroxypyruvate reductase A	polymer	318	36096.1	1	UniProt (A0A140NAE3) Pfam (PF02826) UniProt (by SIFTS) (P75913) In PDB	Escherichia coli (strain B / BL21-DE3)	2-ketoacid reductase
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	A	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
4	A	GLYOXYLIC ACID	non-polymer		74.0	1	Chemie (GLV)
5	A	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
6		water	water		18.0	118	Chemie (HOH)

Sequence modifications

A: 1 - 312 (UniProt: A0A140NAE3)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Phe 45	Trp 45	engineered mutation
Ser 46	His 46	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36096.1
Non-Polymers*	Number of molecules	6
	Total formula weight	978.5
All*	Total formula weight	37074.6

*Water molecules are not included.