6OTZ

Structure of HIV-1 Reverse Transcriptase (RT) in complex with dsDNA and (+)FTC-TP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reverse transcriptase/ribonuclease H	polymer	558	64309.6	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)	p66 RT
2	B (B)	p51 RT	polymer	452	52748.4	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)
3	C (P)	DNA Primer 20-mer	polymer	21	6460.2	1		synthetic construct
4	D (T)	DNA template 27-mer	polymer	27	8408.4	1		synthetic construct
5	E, J, K (A, P, T)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	F, G (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	H (A)	[[(2~{S},5~{R})-5-(4-azanyl-5-fluoranyl-2-oxidanylidene-pyrimidin-1-yl)-1,3-oxathiolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate	non-polymer		487.2	1	Chemie (N8G)
8	I (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	L, M, N (A, B, P)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

A: 1 - 558 (UniProt: P04585)

PDB	External Database	Details
Cys 258	Gln 845	engineered mutation
Ser 280	Cys 867	engineered mutation

B: 1 - 440 (UniProt: P04585)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	131926.7
Non-Polymers*	Number of molecules	7
	Total formula weight	916.1
All*	Total formula weight	132842.7

*Water molecules are not included.