6OS7

E. coli fumarase mutant - R126A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fumarate hydratase class II	polymer	472	51153.4	2	UniProt (P05042) Pfam (PF00206) Pfam (PF10415)	Escherichia coli K-12	Fumarase C,Aerobic fumarase,Iron-independent fumarase
2	C, F (A, B)	CITRATE ANION	non-polymer		189.1	2	Chemie (FLC)
3	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H (A, B)	water	water		18.0	585	Chemie (HOH)

Sequence modifications

A, B: 1 - 467 (UniProt: P05042)

PDB	External Database	Details
Ala 126	Arg 126	engineered mutation
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102306.8
Non-Polymers*	Number of molecules	4
	Total formula weight	562.4
All*	Total formula weight	102869.1

*Water molecules are not included.