6OS2

Structure of synthetic nanobody-stabilized angiotensin II type 1 receptor bound to TRV026

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Type-1 angiotensin II receptor,Soluble cytochrome b562 BRIL fusion protein	polymer	425	48514.9	1	UniProt (P30556) UniProt (P0ABE7) Pfam (PF00001) Pfam (PF07361) In PDB	Homo sapiens (Human)	AT1AR,AT1BR,Angiotensin II type-1 receptor,AT1,Cytochrome b-562
2	D	Nanobody Nb.AT110i1_le	polymer	128	13796.1	1		synthetic construct
3	B	TRV026 peptide	polymer	8	905.0	1		synthetic construct
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	6	Chemie (OLC)
6	A	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
7		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A: 2 - 226 (UniProt: P30556)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

A: 227 - 1226 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 232	Met 29	engineered mutation

A: 1227 - 1319 (UniProt: P30556)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	63216.1
Non-Polymers*	Number of molecules	8
	Total formula weight	2747.1
All*	Total formula weight	65963.2

*Water molecules are not included.